Fig. 5: SlNAP1 directly targets SlGA2ox3, SlPAL3, and SlNCED1. | Horticulture Research

Fig. 5: SlNAP1 directly targets SlGA2ox3, SlPAL3, and SlNCED1.

From: Transcriptomic and genetic approaches reveal an essential role of the NAC transcription factor SlNAP1 in the growth and defense response of tomato

Fig. 5

a Electrophoretic mobility shift assay. A SlNAP1-His fusion protein was incubated with biotin-labeled wild-type (SlGA2ox3-wt) or mutant (SlGA2ox3-mut) SlGA2ox3 oligos, wild-type (SlPAL3-wt) or mutant (SlPAL3-mut) SlPAL3 oligos and wild-type (SlNCED1-wt) or mutant (SlNCED1-mut) SlNCED1 oligos. Proteins purified from the empty vector (EV) were used as negative controls in this experiment. b Chromatin immunoprecipitation assay. Samples from wild-type and 35S:SlNAP1-HA tomato plants were precipitated using anti-HA antibodies. A control reaction with goat anti-mouse IgG was simultaneously processed. The ChIP results are shown as percentages of the input DNA. The results are shown as averages ± SDs, n = 3. The different letters indicate significant differences (P < 0.05, Tukey’s test)

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