Fig. 2
From: Roles of SlETR7, a newly discovered ethylene receptor, in tomato plant and fruit development
Tomato contains a seventh ethylene receptor. a Schematic structure of SlETR7 protein. Three conserved transmembrane domains are predicted at N-terminus followed by GAF, kinase, and receiver domains using TMpred and SMART tools. b Phylogenetic tree analysis of ETR proteins in Arabidopsis, tomato, and rice. The scale bar presents the substitution per amino acid based on bootstrap method (B = 1000 replications). The accessions of ETR proteins used in this analysis are: AtETR1 (NP_176808), AtERS1 (NP_181626), AtERS2 (NP_001323287), AtEIN4 (NP_187108), AtETR2 (XP_002883407), SlETR1 (NP_001234149), SlETR2 (NP_001234153), SlETR3 (NP_001233894), SlETR4 (NP_001234205), SlETR5 (NP_001234212), SlETR6 (NP_001234150). The amino acid sequence was translated from the coding region (Supplementary S2C). c Ethylene binding assays were conducted on P. pastoris cells expressing either GST alone (GST) as a negative control or expressing the first 130 amino acids of SlETR7 fused to GST (ETR7[1–130]GST). Data represent the mean ± SD, n = 3. The “***” indicates a significant difference using a t-test (P < 0.001) between the binding levels with 14C-ethylene alone versus 14C-ethylene plus excess of 12C-ethylene
