Fig. 2: Changes in the levels of accumulated ROS and activities of ROS-scavenging enzymes in apple leaves under heat stress. | Horticulture Research

Fig. 2: Changes in the levels of accumulated ROS and activities of ROS-scavenging enzymes in apple leaves under heat stress.

From: MdATG18a overexpression improves basal thermotolerance in transgenic apple by decreasing damage to chloroplasts

Fig. 2

a In situ accumulation of H2O2 and superoxide radical (O2−) before (left panels) and after (right panels) heat treatment revealed by DAB and NBT staining, respectively. Quantitative measurement of b H2O2 and c O2− concentrations in apple leaves treated with and without high temperature. Activities of d superoxide dismutase (SOD), e peroxidase (POD) and f catalase (CAT) after 4 h of heat treatment. Data are shown as the means of three replicates with SEs. Different letters indicate significant differences between treatments, according to one-way ANOVA and Tukey’s multiple range test (P < 0.05).

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