Fig. 6: Detection of CmWRKY33.1 transgenic lines and phenotypes after inoculation with A. alternata. | Horticulture Research

Fig. 6: Detection of CmWRKY33.1 transgenic lines and phenotypes after inoculation with A. alternata.

From: A temporal gene expression map of Chrysanthemum leaves infected with Alternaria alternata reveals different stages of defense mechanisms

Fig. 6

a Electrophoresis detection of transgenic lines. 3–68: Overexpressing lines; + Positive control (Plasmid DNA), − Negative control (H2O), WT Wild-type ‘Jinba’. Up: PCR primers CmWRKY33.1-ID-F1/R1 were used for amplification of the pMDC43-CmWRKY33.1 vector in the transformation lines. Down: PCR primers CmWRKY33.1-ID-F2/R2 were used for amplification of the pMDC43-CmWRKY33.1 vector in the transformation lines. b Relative expression of CmWRKY33.1 in the overexpression lines. c Phenotypic differences of wild-type and CmWRKY33.1-overexpressing lines. Bar = 10 mm

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