Table 1 Kinetic data of recombinant UGT74AF3 for HDMF

From: UGT74AF3 enzymes specifically catalyze the glucosylation of 4-hydroxy-2,5-dimethylfuran-3(2H)-one, an important volatile compound in Camellia sinensis

Proteins

Substrate

KM (µM)

Vmax (nKat/mg)

Kcat/KM (µM−1 s−1)

UGT74AF3a

HDMF

1.59 ± 0.19

4.23 ± 0.02

40.6

UGT74AF3b

HDMF

5.14 ± 1.58

11.38 ± 2.18

28.7

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