Fig. 3: Detection of protein–protein interaction between MetE and SAHH. | Horticulture Research

Fig. 3: Detection of protein–protein interaction between MetE and SAHH.

From: Application of an antibody chip for screening differentially expressed proteins during peach ripening and identification of a metabolon in the SAM cycle to generate a peach ethylene biosynthesis model

Fig. 3: Detection of protein–protein interaction between MetE and SAHH.

a Yeast two-hybrid analysis of the interaction between MetE and SAHH. Yeast transformants were grown on QDO medium (SD-Ade/-His/-Leu/-Trp) (left panel) or QDO medium supplemented with X gal (right panel). pGBKt7/pGADT7-T is a positive control. b BIFC analysis of the interaction between MetE and SAHH. The combined constructs (top to bottom) are PSPYNE-35S-MetE/PSPYCE-35S-SAHH, PSPYNE-35S/PSPYCE-35S (negative control), and PSPYNE-35S-bZIP63/PSPYCE-35S-bZIP63 (positive control). Bar = 32 μm

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