Fig. 3

CsWAKL08 localizes to the plasma membrane. a The plasmid used for transient expression; 35S, cauliflower mosaic virus 35S promoter; NOS, NOS terminator; NPTII, NptII gene; GFP, GFP gene; LB: left border; RB: right border. GFP fluorescence of baseline onion epidermal cells (b) and after plasmolysis (c) were taken as the control. Transient expression was measured based on the fluorescence of CsWAKL08-GFP at baseline (d) and after plasmolysis (e). In (b)–(e), scale bar = 200 μm; fields of view are shown as dark field, bright field, and merged images