Fig. 5 | Horticulture Research

Fig. 5

From: CsWAKL08, a pathogen-induced wall-associated receptor-like kinase in sweet orange, confers resistance to citrus bacterial canker via ROS control and JA signaling

Fig. 5

Assessment of Xcc responses in the CsWAKL08-silenced plants. a Plasmid used for RNAi assays; 35S, cauliflower mosaic virus 35S promoter; NOS, NOS terminator; NPTII, NptII gene; LB: left border; RB: right border. Transgenic plants were confirmed by PCR (b) and GUS staining (c). d CsWAKL08 expression as assessed via qRT-PCR with CsActin (GenBank: GU911361.1) as a normalization control. e Phenotypes of the RNAi transgenic plants. Scale bar = 100 mm. f Xcc-induced disease symptoms of the transgenic and CK plants at 10 dpi. The scale bar for the leaves was 20 mm, while for the lesions, it was 5 mm. For analysis of disease resistance, lesion sizes (LS) (g) and disease index (DI) (h) were analyzed. In (d), (g) and (h), *P < 0.05, significant difference; **P < 0.01, extremely significant difference; data are the mean ± SD. i CBC resistance assay of the RNAi plants using the infiltration method. Xcc-induced disease symptoms were observed at 10 dpi

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