Fig. 6: bHLH3 is an indispensable factor that activates flavonoid biosynthesis. | Horticulture Research

Fig. 6: bHLH3 is an indispensable factor that activates flavonoid biosynthesis.

From: Abnormal expression of bHLH3 disrupts a flavonoid homeostasis network, causing differences in pigment composition among mulberry fruits

Fig. 6

a Dual-luciferase reporter assay showing that activation of the promoters of CHS2, CHI, F3H, CYP75B1, DFR, and UFGT requires participation of bHLH3. the data represent averages of four experiments. Asterisks (*) indicate the statistical significance of the difference between the experimental and the control groups. (Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001). b Connection network among transcription factors, anthocyanin pathway genes, and anthocyanins. c The positive transgenic lines were determined by semiquantitative RT-PCR. AtActin served as an internal standard. d Restored seed pigmentation in the Arabidopsis tt8 mutant overexpressing bHLH3. WT, wild-type; tt8-4, Arabidopsis tt8 mutant; bHLH3/tt8, T2 seed of bHLH3-transgenic plant. Scale bars are 1 mm. e Phenotypes of fruits of different mulberry cultivars. F2-R, F2-P, and F2-W are components of the F2 population derived from LJ109 and ZZB. Scale bars are 1 cm. f qRT-PCR analysis of ANS, MYBA, bHLH3, and MYB4 expression levels in ripe fruits of different mulberry cultivars

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