Fig. 4: Molecular features of the major indel sites in AMAT promoters. | Horticulture Research

Fig. 4: Molecular features of the major indel sites in AMAT promoters.

From: A key ‘foxy’ aroma gene is regulated by homology-induced promoter indels in the iconic juice grape ‘Concord’

Fig. 4

DNA sequences of the AMAT promoters, −3310 to −220 bp upstream of the ATG start codon, from 5 different cultivars were illustrated: PN40024, Chardonnay clone I10V1, ‘Caco’, ‘Concord’ and ‘Alba’. “––” represents unspecified numbers of bases. “:” stands for absent bases. The four indels were marked by brackets of different colors: purple for the 426 bp, green for the 26 bp, red for the 42 bp, and aqua for the 3 kb. The direct repeats for each indel were highlighted and marked by solid arrows with corresponding colors. Inverted repeats (IRs) of 4–7 bp were also found for all four indels and were underlined and marked by dashed arrows facing each other. a features of the 3-kb indel. Two pairs of IRs were identified. b features of the 426-bp indel. One pair of IRs and a nested 26-bp indel were identified. The repressor binding motif (‘TTGCTTA’) of CBNAC in the 26-bp indel is highlighted in gray. c features of the 42-bp indel. There are six TATA elements (in orange letters) in the region, but only two of them qualified as TATA consensus sequences (TATAWAW, in bold) and the 42 bp is located between these two consensus TATA sequences

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