Fig. 4: ROS levels were assessed in the different ‘67/3’ seed lots previously characterized for their germination profiles (high-quality and low-quality lots) and response to hydropriming.
From: Molecular dynamics of pre-germinative metabolism in primed eggplant (Solanum melongena L.) seeds
a The experimental design is shown: for each lot, the unprimed samples included dry seeds (UP) as well as rehydrated seeds collected at 2 h of imbibition (UP2), whereas the primed samples included seeds that underwent the HP72 treatment followed by dry-back (HPDB) and seeds collected at 2 h of imbibition (HPDB2). b ROS levels were measured at the selected timepoints using the DCFH-DA fluorescent dye. Values are expressed as mean ± SD of three independent replications with 20 seeds for each replication. Asterisks indicate statistically significant differences determined using Student’s t-test (P < 0.05). DS, dry seed. R.F.U. relative fluorescence unit, ROS reactive oxygen species, DCFH-DA dye 2′,7′-dichlorofluorescein diacetate, SL seed lot
