Fig. 1: Loading of fluo-8/AM into flesh tissue cells.

a Slice of flesh tissue that was not loaded with fluo-8/AM. b Flesh tissue was cut by a cryostat and then fluorescently stained with fluo-8/AM. c Flesh tissue was cut by hand and then fluorescently stained with fluo-8/AM. d Fluo-8/AM loaded into single flesh cells