Fig. 5: Effect of different concentrations of EGTA or La³⁺ on Ca²⁺ concentration in protoplasts.

a Protoplast after loading with fluo-8/AM, followed by the addition of 1 mmol/L EGTA, b 10 mmol/L EGTA, c 10 µmol/L La³⁺, or d 100 µmol/L La³⁺