Fig. 6: Effect of A23187 on Ca2+ concentration in protoplasts. | Horticulture Research

Fig. 6: Effect of A23187 on Ca2+ concentration in protoplasts.

From: Loading calcium fluorescent probes into protoplasts to detect calcium in the flesh tissue cells of Malus domestica

Fig. 6

a Control protoplast loaded with fluo-8/AM. b Protoplast after loading with fluo-8/AM, followed by the addition of 5 µmol/L A23187 or c 50 µmol/L A23187; d protoplast after loading with fluo-8/AM, followed by the addition of 100 µmol/L La3+. e Protoplast after loading with fluo-8/AM, followed by the addition of 5 µmol/L A23187 and 100 µmol/L La3+. f 50 µmol/L A23187 and 100 µmol/L La3+. g Statistical analysis of fluorescence intensity in the protoplasts. *** Indicates a significant difference (P < 0.001, Student’s t-test). * Indicates a significant difference (P < 0.05, Student’s t-test). Vertical bars indicate ± SE. Each data point represents the mean of 10 protoplasts. a–f Representative fluorescence images of protoplasts

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