Fig. 7: Fluorescence changes in Ca2+ in the same protoplast within 25 min. Pictures were taken every 5 min. | Horticulture Research

Fig. 7: Fluorescence changes in Ca2+ in the same protoplast within 25 min. Pictures were taken every 5 min.

From: Loading calcium fluorescent probes into protoplasts to detect calcium in the flesh tissue cells of Malus domestica

Fig. 7

a Protoplast loaded with fluo-8/AM (Control). b The calcium ion fluorescence was observed for 5 min, and then 100 µmol/L La3+ was added. c The calcium ion fluorescence was observed for 5 min, and then 10 mmol/L EGTA was added. d Calcium ion fluorescence was observed for 5 min, and then 5 µmol/L A23187 was added. e The calcium ion fluorescence was observed for 5 min, and then 1 µmol/L ionomycin was added. Vertical bars indicate ± SE. Each data point represents the mean of three protoplasts. a–e Representative fluorescence images of protoplasts

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