Fig. 5: CmMYB2 interacts with CmBBX24 both in vitro and in vivo. | Horticulture Research

Fig. 5: CmMYB2 interacts with CmBBX24 both in vitro and in vivo.

From: Regulation of flowering time in chrysanthemum by the R2R3 MYB transcription factor CmMYB2 is associated with changes in gibberellin metabolism

Fig. 5

a A yeast two-hybrid assay showing protein interactions. -TL, SD/-Trp/-Leu; -TLHA, SD/-Trp/-Leu/-Ade/-His; -TLHA + X-α-gal, SD/-Trp/-Leu/-Ade/-His/X-α-gal; vec, empty vector; CmBBX24boxs, two B-boxes in the N-terminus of CmBBX24; CmBBX24box1, the first B-box in the N-terminus of CmBBX24; CmBBX24box2, the second B-box in the N-terminus of CmBBX24; pGBKT7-53 and pGADT7-T served as positive controls, and pGBKT7-Lam and pGADT7-T served as negative controls. b A BiFC assay was used to confirm the interaction between CmMYB2 and CmBBX24, given the results of the yeast two-hybrid assay. YFP: yellow fluorescent protein, Marker: nuclear localization shown by RFP activity, DIC: bright field image, Merge: overlay of YFP, RFP and bright field images

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