Fig. 6: Silencing CaNHL4 in pepper enhances the susceptibility to P. capsici, TMV and P. syringae.

a The expression level of CaNHL4 and TRV CP was quantified by qPCR at 14 days after inoculation with TRV1 + TRV:CaNHL4 or TRV:00. b and c Silencing CaNHL4 enhances the infection of P. capsici. Disease lesion size was measured and is presented. d–f Silencing CaNHL4 promotes the movement and accumulation of TMV-GFP. The expression of TMV-CP and accumulation of TMV-GFP were measured. g, h Silencing CaNHL4 promotes the infection of P. syringae. Lesion size was measured and is presented. All the experiments were repeated three times and showed similar results. The values represent means ± standard errors (SEs) of three biological replications. The statistical analyses were performed using Student’s t-test (∗0.01 < P < 0.05; ∗∗0.001 < P < 0.01; ∗∗∗P < 0.001)