Fig. 2: Analysis of the interactions between CsPGR5a and candidate proteins. | Horticulture Research

Fig. 2: Analysis of the interactions between CsPGR5a and candidate proteins.

From: The key cyclic electron flow protein PGR5 associates with cytochrome b6f, and its function is partially influenced by the LHCII state transition

Fig. 2

a Yeast two-hybrid assay analysis. CsPGR5a was fused to the DNA-binding domain (PGR5a-BD), while the candidate proteins were fused to the activation domain (PetC-AD, LHCAP4-AD, PsaH-AD, NDH5-AD, atpB-AD, PP2C25-AD, PsaK-AD, Lhcb3-AD, FDA-AD, PsaG-AD, CP12-2-AD, PsbS-AD, PGRL1A-AD, PGRL1B-AD, and PGR5b-AD). pGBKT7-53+pGADT7-T and pGBKT7-Lam + pGADT7-T were used as positive and negative controls, respectively. b BiFC assay of the interactions of PetC, Lhcb3, and PsaH with PGR5a. Plasmids encoding fusion constructs with the N- or C-terminal parts of YFP (PGR5a-YFPn, PetC-YFPc, Lhcb3-YFPc, and PsaH-YFPc) were separately transiently expressed in Nicotiana benthamiana leaves. The yellow signals indicate YFP fluorescence; the magenta signals indicate chloroplast autofluorescence

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