Fig. 2: Purity evaluation of isolated mitochondria.
From: Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit
a Western blot analysis of mitochondrial enriched fractions (mito) and total pulp protein extracts (pulp) of different citrus species. A total of 10 μg of mitochondrial proteins and pulp proteins were separated by 10% SDS-PAGE and blotted onto PVDF membranes. Blots were performed using different marker antibodies against VDAC1 (mitochondrial outer membrane), SHMT (mitochondrial matrix), UGPase (cytoplasm), RbcL (plastid), and Cat (peroxisome). Uncropped blot images are shown in Supplementary Fig. S6. b–e Transmission electron microscopy (TEM) images of isolated mitochondria from (b) satsuma mandarin, (c) ponkan mandarin, (d) sweet orange, and (e) shatian pummelo. Bars = 1 μm
