Fig. 5: LlWRKY39 activates the expression of LlMBF1c.

a Transient expression of LlWRKY39 in lily increased the expression of LlMBF1c. Each bar indicates the mean ± SD of three repeated experiments. Significant differences between the control and transient overexpression plants were determined by t test (*P < 0.05). b The sequence of the LlMBF1c promoter and its W-box mutated version; the red label indicates the W-box element. c Yeast one-hybrid assay. The interaction between LlWRKY39 and W-box elements was determined in SD-LWH medium with different concentrations of 3-AT. d Schematic diagram of the LlMBF1c promoter; the probe sequence is shown below the diagram. e LlWRKY39-HIS complex binds to the W-box in EMSA. The term 250× indicates the usage of excess unlabeled probe as a competitor, and “+” and “−” indicate its presence and absence, respectively. f The schematic diagram of the effector and reporter. The 836-bp fragment of the LlMBF1c promoter was used in this assay. g Bright field and dark field images of N. benthamiana leaves in the transient expression assays. h The ratio of LUC/REN. 1: mixed bacterial solutions of pGreenII-62-SK and pGreenII-0800-LUC (2:1); 2: mixed bacterial solutions of pGreenII-62-SK-LlWRKY39 and pGreenII-0800-LUC (2:1); 3: mixed bacterial solutions of pGreenII-62-SK and pGreenII-0800-proLlMBF1c-LUC (2:1); 4: mixed bacterial solutions of pGreenII-62-SK-LlWRKY39 and pGreenII-0800-proLlMBF1c-LUC (2:1). Each bar indicates the mean ± SD of three repeated experiments (*P < 0.05, t test)