Fig. 5: Functional verification of MSTRG.19915 and BrMAPK15 in downy mildew resistance using VIGS.
From: Identification of long noncoding RNAs involved in resistance to downy mildew in Chinese cabbage
a Schematic diagram of the genomic location, transcription direction, and locations of designed primers for MSTRG.19915 and BrMAPK15. Specific primers (avoiding overlapping and conserved regions) were designed to ensure the silencing specificity and reliability for each target. b FPKM values of MSTRG.19915 and BrMAPK15 in the sequenced samples. c Relative expression levels of MSTRG.19915 and BrMAPK15 in sequenced samples according to qRT-PCR. d Change in the expression level of BrMAPK15 after silencing BrMAPK15 in 12–85; e Change in the expression level of MSTRG.19915 after silencing MSTRG.19915 in 91–112; f Change in the expression level of BrMAPK15 after silencing MSTRG.19915 in 91–112; g The phenotypes of 12–85, the BrMAPK15-silenced line, 91–112 and the MSTRG.19915-silenced line were investigated 5 days after H. brassicae inoculation. The PDS silencing line was used as a positive control for the VIGS method. The values represent the means ± SDs (n = 3) of three biological replicates
