Fig. 6: Functional analysis of MSTRG.19915 and BrMAPK15 in downy mildew resistance by agroinfiltration-mediated transient expression.

Relative expression levels of MSTRG.19915 (a) and BrMAPK15 (c) overexpressed in 12–85 and 91–112 cells, respectively, were measured. The values represent the means ± SDs (n = 3) of three biological replicates. The expression of each gene before infiltration was defined as 1.0. The percentages of susceptible infiltrated cotyledons in MSTRG.19915 overexpression lines (b) and BrMAPK15 lines (d) after inoculation were investigated. Cotyledons of 12–85 and 91–112 infiltrated with empty vectors were used as the controls. The values represent the means ± SDs of three independent experiments. Each experiment involved 30–50 cotyledons. e The phenotypes of cotyledons infiltrated with MSTRG.19915 and BrMAPK15 were observed at 3 days after agroinfiltration. Leaves infiltrated with empty vectors were used as negative controls. The images in small red squares are magnified. The values represent the means ± SDs (n = 3) of three biological replicates