Fig. 1: Analysis of Fra a 1 proteins in strawberry fruits along ripening and in transgenic lines.

a Photographs of fruits representative of green, white, turning and red stages of fruit development, and western blot analysis of extracts from strawberry receptacles at these stages, using anti-Fra a 1.02 antibodies. FaFra a 1.01, FaFra a 1.02, and FaFra a 1.03 recombinant proteins, with a 6X His tag, purified from E. coli were used as controls. Equal amounts of total protein extracts from strawberry were loaded. b Western blot of fruit extracts from transgenic lines L4, L5, L6, at three developmental stages, using anti-Fra a 1.02 antibodies. Controls correspond to plants transformed with the empty vector. A positive control of ripe fruit extract of the cv. Camarosa is included. Membranes stained with Coomassie Brilliant Blue (CBB) are also shown for each stage sample.