Fig. 2: Subcellular localization of AaBAM3.1-GFP and BAM activity in pGEX4T-1. | Horticulture Research

Fig. 2: Subcellular localization of AaBAM3.1-GFP and BAM activity in pGEX4T-1.

From: The AaCBF4-AaBAM3.1 module enhances freezing tolerance of kiwifruit (Actinidia arguta)

Fig. 2: Subcellular localization of AaBAM3.1-GFP and BAM activity in pGEX4T-1.

a Tobacco leaves were injected with the control (35S-GFP) or recombinant plasmid (35S-AaBAM3.1-GFP) and visualized under a confocal microscope. GFP imaging and autofluorescence are shown. b SDS-PAGE analysis of the expression of the recombinant protein in E. coli. The total proteins from bacteria and the purified recombinant protein from the soluble crude extract were separated via 10% SDS-PAGE and stained with Coomassie brilliant blue. M, protein size marker (15–150 kDa). Lane 1 contains proteins from uninduced cells, and lane 2 contains proteins from induced cells. The OD540 results obtained using a BAM enzyme kit showed that the recombinant protein presented enzymatic activity

Back to article page