Fig. 2: Characterization of LcBZR1 and LcBZR2. | Horticulture Research

Fig. 2: Characterization of LcBZR1 and LcBZR2.

From: Brassinosteroids suppress ethylene-induced fruitlet abscission through LcBZR1/2-mediated transcriptional repression of LcACS1/4 and LcACO2/3 in litchi

Fig. 2

a Amino acid sequence alignment of LcBZR1/2 with homologs from banana (Ma, Musa acuminate L.), rice (Os, Oryza sativa L.) and Arabidopsis (At, Arabidopsis thaliana). b Phylogenetic analysis of LcBZR1/2 with homologs from banana (Ma, Musa acuminate L.), rice (Os, Oryza sativa L.), tomato (Sl, Solanum lycopersicum) and Arabidopsis (At, Arabidopsis thaliana). c Subcellular localization of LcBZR1/2 in tobacco leaves. LcBZR1/2 fused to GFP and the positive control pEAQ-GFP were transformed into tobacco leaves. After 48 h of incubation, GFP signals were observed under a confocal laser scanning microscope. The nucleus is indicated by 4,6-diamidino-2-phenylindole (DAPI) staining. Merged images indicate the colocalization of DAPI and GFP signals. Scale bars indicate 25 µm. d Transcriptional repression ability of LcBZR1/2 in tobacco leaves. The transcriptional activity of LcBZR1/2 was tested by the dal-luciferase reporter assay by detecting the ratio of LUC to REN. The SE was calculated from six replicates. **Significant differences in values according to Student’s t test (P < 0.01).

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