Fig. 5: CsMYB4 and CsMYB7 repressors mediated shading-induced repression of flavonol biosynthesis. | Horticulture Research

Fig. 5: CsMYB4 and CsMYB7 repressors mediated shading-induced repression of flavonol biosynthesis.

From: CsbZIP1-CsMYB12 mediates the production of bitter-tasting flavonols in tea plants (Camellia sinensis) through a coordinated activator–repressor network

Fig. 5: CsMYB4 and CsMYB7 repressors mediated shading-induced repression of flavonol biosynthesis.

a, c Effect of shading (SD) on CsMYB4 and CsMYB7 expression in tea leaves compared with regular light (LT). b, d qRT-PCR data show the expression profiles of CsMYB4 and CsMYB7 in different stages. e, f Nuclear localization of GFP-CsMYB4 and GFP-CsMYB7 fusion in tobacco leaf epidermal cells. Bar = 50 µm. g Binding of CsMYB4 and CsMYB7 to the promoters of CsFLS and CsUGT78A14 in Y1H assays. h Interactions among CsMYB12, CsMYB4, and CsMYB7 in Y2H assays. i Constructions of reporter and effector expression vectors for dual luciferase assays. j Transactivation of CsMYB12, CsMYB4, and CsMYB7 individually or in combination on the promoter activity of CsFLS in the luciferase reporter assay. k Effect of UV-B radiation on the expression of CsMYB4, CsMYB7, and CsbZIP1. Differences between shading (SD) and light (LT) (control) were analyzed. Data were from three independent experiments and expressed as the means ± S.D. (n = 3). Differences comparison with the control were analyzed by two-tailed Student’s t test, *p < 0.05; **p < 0.01

Back to article page