Fig. 1: CRISPR/Cas9-mediated mutation in DPL. | Horticulture Research

Fig. 1: CRISPR/Cas9-mediated mutation in DPL.

From: CRISPR/Cas9-mediated targeted mutation reveals a role for AN4 rather than DPL in regulating venation formation in the corolla tube of Petunia hybrida

Fig. 1

a Diagram of the target site in the genomic region of DPL. The blue line indicates the PAM (NGG) motif. The blue boxes and black lines indicate exons and introns, respectively. b Targeted mutagenesis in DPL for the dpl-M3, -M4, and -M5 lines. The target sequence is shown in blue letters, for which red dashes indicate deletions, while red letters indicate insertions. c–e Flower buds of MD and a representative line dpl-M3; flower buds in c and d are from plants cultured at 25 °C, and those in e are from plants treated at 12 ± 2 °C (cold stress conditions). Scale bars = 1 cm

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