Fig. 2: Gene cloning, sequence variation and structure of the SlRCM1 gene.

a Phenotypes of leaves and fruits from the wild-type and rcm1 mutant plants and their F₁ progeny. Scale bars, 2 cm. b Chlorophyll content of mature leaves and MG-stage fruits from the wild type and rcm1 mutant and their F₁ progeny. The data are presented as the means ± SDs (n = 3). The means followed by different letters indicate statistical significance at P = 0.05. c Differences in allele frequencies between 25 lines with green fruits and 25 lines with yellowish fruits in the F₂ population. The X axis represents the 12 chromosomes of tomato. The Y axis represents the difference in allele frequencies (SNP values) between the two pools. The red line represents the 95% confidence interval. The SNPs associated with the 95% confidence interval are located between SL2.50ch08_1 and SL2.50ch08_1010000. The blue line represents the average value of the ΔSNP index per 1 Mb window. d The fragment containing SNP (SL2.50ch08_16268) was subjected to PCR amplification and Sanger sequencing. The red letters indicate the bases of M82 (T), Ligeer 87-5 (T), rcm1 (A) and members of the hybrid F₂ generation (A) with reduced chlorophyll at SL2.50ch08_16268. “-“ indicates the antisense strand of the genome. e Structure of the SlRCM1 gene. The black lines represent introns and untranslated regions. The white boxes represent exons. The T (WT)-to-A (rcm1) substitution in the second exon of SlRCM1 caused a conversion from Tyr (WT) to a stop codon (rcm1)