Fig. 2: Map-based cloning of Su.
From: Mutation of SlARC6 leads to tissue-specific defects in chloroplast development in tomato
A Mapping of Su by BSA-seq analysis. Significantly associated single-nucleotide polymorphisms (SNPs) were found between 63 and 66 Mb of chromosome 4. B Rough mapping of Su. The black line represents chromosome 4; characters above the line show markers used in the rough mapping; characters behind the line show the number of recombinations. The interval between markers M17 and M31 is 2.14 Mb. A total of 456 individuals with albinic stems were used in the rough mapping. C Fine mapping of Su. The interval between markers M28 and M220 is 531.8 Kb. A total of 515 individuals with white stems were used in the fine mapping. D Candidate genes in the target region. Boxes with arrows represent open reading frames (ORFs) in the target region according to the tomato genome (SL2.4). ORFs above the line are located in the positive strand of the chromosome, and ORFs below the line are in the antisense strand. The red box with arrow shows the target gene. E Gene structure of ORF8. The black line shows the coding sequence of ORF8, which encodes 819 amino acids. The hatched boxes represent domains of ORF8. The red lightning bolt shows the mutation site of ORF8 in LA0628
