Fig. 3: Functional verification of ORF8 by virus-induced gene silencing (VIGS).
From: Mutation of SlARC6 leads to tissue-specific defects in chloroplast development in tomato
A–I The phenotype of VIGS-ORF8 plants. A–C Plants injected with Agrobacterium containing empty TRV2 were used as the negative control. D–F VIGS-PDS plants were used as the positive control. G–I The VIGS-ORF8 plants. J Protoplasts from the mesophyll of the negative plants (the left channel) and VIGS-ORF8 plants (the right channel). K Protoplasts from the stem of the negative plants (the left channel) and VIGS-ORF8 plants (the right channel). The panels filled with crosses show that there are no undivided chloroplasts in the negative plants. Both undivided and divided chloroplasts could be found in the stems of VIGS-ORF8 plants
