Fig. 4: Functional verification of ORF8 by CRISPR. | Horticulture Research

Fig. 4: Functional verification of ORF8 by CRISPR.

From: Mutation of SlARC6 leads to tissue-specific defects in chloroplast development in tomato

Fig. 4

A Slorf8 edited alleles found in the transgenic plants. Blue boxes represent exons, black boxes represent introns, and pink boxes represent UTR regions. The sgRNA and PAM sequence are shown. Two homozygous edited lines were used in this study, one showing a G insertion and one a G deletion. B Wild-type (WT) plants were used as the negative control. C The phenotype of the Slorf8 ko-2 line. Bars: 1 cm

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