Fig. 4: CRISPR/Cas9 knock out confirms Solyc12g006860 as the GLOBE gene. | Horticulture Research

Fig. 4: CRISPR/Cas9 knock out confirms Solyc12g006860 as the GLOBE gene.

From: Identification and characterization of GLOBE, a major gene controlling fruit shape and impacting fruit size and marketability in tomato

Fig. 4

A cDNA sequence of Solyc12g006860 showing the two guide RNAs (1, yellow shade; and 2, gray shade) and the two deletions caused in the gene (the first of 59 bp, the second of 3 bp). The inverted red triangle shows the location of the natural globe mutation (insertion of a thymine). B Phenotypic measurements of fruit shape index and pedicel length in Fla. 8059 (WT) and CRISPR/Cas9 knocked out plants (MUT). MUT and WT are significantly different with P < 0.001, error bars represent the standard error of the mean (n = 5 plants; each observation was derived from the average of at least 3 fruits). C Sliced fruit of each of the wild-type (upper) and mutant plants (lower). Scale bar is 2.5 cm

Back to article page