Fig. 4: Analysis of the basic properties of AeADF1. | Horticulture Research

Fig. 4: Analysis of the basic properties of AeADF1.

From: Hyperoside promotes pollen tube growth by regulating the depolymerization effect of actin-depolymerizing factor 1 on microfilaments in okra

Fig. 4

A The homology model was created using the Phyre2 website (www.sbg.bio.ic.ac.uk/phyre2). The structures of AeADF1 and homologous ADF1 proteins were predicted with a >90% confidence interval. B Conserved protein domains of AeADF1 and homologous ADF1 proteins were predicted using the SMART database (http://smart.embl-heidelberg.de/) to confirm that they are members of the ADF family. C Semiquantitative reverse transcription-PCR analysis of AeADF1 in the pollen, petals, styles, and filaments of control and hyperoside-treated flowers. A. esculentus actin was used as a control. D qRT-PCR analysis of AeADF1 expression in the pollen, petals, styles, and filaments of control and hyperoside-treated flowers. Each treatment had three biological replicates, and error bars display the standard error of the sample. *P < 0.05 (Student’s t test)

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