Fig. 6: AeADF1 dynamically severs F-actin under hyperoside treatment.

A After adding 0.1 μM AeADF1, 50 μM hyperoside, or both, prefabricated rhodamine-labeled F-actin was immediately observed through a total internal reflection fluorescence microscope. Bar = 5 μm. B F-actin-severing frequencies (breaks μm−1 s−1) were measured in (A). Each treatment had three biological replicates, and error bars display the standard error of the sample. *P < 0.05 (Student’s t test)