Fig. 7: AeADF1 severs F-actin under hyperoside treatment.

Actin filaments (polymerized from 0.5 μM G-actin) were visualized by staining with 0.5 μM Alexa-488 phalloidin. A F-actin was incubated with 0.1 μM AeADF1 and various concentrations of hyperoside. B The average length of individual actin filaments in the reactions from (A). C F-actin was incubated with 50 μM hyperoside and various concentrations of AeADF1. D The average length of individual actin filaments in the reactions from (C). In B, D, each treatment had three biological replicates, and error bars display the standard error of the sample. *P < 0.05 (Student’s t test). Scale bar = 5 μm