Fig. 6: Distribution of SlACR11A alleles in tomato.
From: Interactions between ShPP2-1, an F-box family gene, and ACR11A regulate cold tolerance of tomato
a Diagram of the seed germination experiment. Germination at 15 °C was used to distinguish between cold-sensitive and cold-tolerant accessions. A temperate of 25 °C was considered an optimal condition (control) for seed germination. b Percentage of wild, CER, and BIG accessions with particular relative germination indices (rGI). c Variation in rGIs among the wild, CER, and BIG accessions. d Distribution of rGIs for 177 tomato accessions harboring either the SlACR11AG or SlACR11AA allele. For b, c, and d, seeds were germinated at either 25 °C or 15 °C for 7 days. The rGI was calculated using the germination index at 15 °C relative to 25 °C. Approximately 100 seeds of each accession were used in each germination assay. P-values were calculated using two-tailed Student’s t tests. e Gene structure of SlACR11A. The G/A SNP in the first exon causes an Ala-16 (A) to Thr (T) substitution. The boxes and lines represent exons and introns, respectively. f Seed germination of cold-tolerant and cold-sensitive accessions. Three accessions harbored the SlACR11AG allele, and three accessions harbored the SlACR11AA allele. Approximately 50 seeds of each accession were used in each germination assay, which was conducted at either 25 °C or 15 °C for 15 days
