Fig. 6: Silencing of GhSUS2 represses starch synthesis in corms.

A Expression of GhSUS2 in silenced corms. B qRT-PCR results showing an increase in the GhCIN transcript level in GhSUS2-silenced corms. C Downregulation of GhAPS expression in GhSUS2-silenced corms. D Decreased sucrose content in GhSUS2-silenced corms. E Accumulation of glucose in GhSUS2-silenced corms. F Repression of starch synthesis in GhSUS2-silenced corms. The enzyme activity of sucrose synthase (G) or soluble starch synthase (SSS; I) was decreased in GhSUS2-silenced corms. H The enzyme activity of invertase was increased in GhSUS2-silenced corms. Averages of three to five biological replicates ± SDs are shown. Significant differences were determined by the two-sided t-test. The P value is indicated above the black line