Fig. 5: Interactions between SlBBX20 and SlCSN5-2. | Horticulture Research

Fig. 5: Interactions between SlBBX20 and SlCSN5-2.

From: SlBBX20 interacts with the COP9 signalosome subunit SlCSN5-2 to regulate anthocyanin biosynthesis by activating SlDFR expression in tomato

Fig. 5

a Interaction between SlBBX20 and SlCSN5-2 in yeast two-hybrid experiments. The BD-S1BBX20 and AD-S1CSN5-257-367 (residues 57 to 367) plasmids were cotransformed into yeast strain AH109. b Interaction between SlBBX20 and SlCSN5-2 in coimmunoprecipitation assays. SlBBX20-HA and SlCSN5-2-FLAG were simultaneously introduced into tobacco protoplasts. The proteins were immunoprecipitated using anti-FLAG matrix beads and analyzed by western blotting. c Interaction between SlBBX20 and SlCSN5-2 in BiFC assays. The pHBT-SlBBX20-nYFP and pHBT-SlCSN5-2-cYFP plasmids were cotransformed into Arabidopsis protoplasts. The pHBT-SlBBX20-nYFP and cYFP plasmids or pHBT-SlCSN5-2-cYFP and nYFP plasmids as a negative control were used. YFP fluorescence was observed using confocal laser scanning microscopy. d Subcellular localization of SlCSN5-2. SlCSN5-2 without a stop codon was cloned into pHBT-GFP. Tobacco protoplasts were extracted and cotransformed with plasmids encoding SlCSN5-2-GFP and a nuclear marker fused to RFP

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