Fig. 7: SlCSN5-2 regulates accumulation of the SlBBX20 protein. | Horticulture Research

Fig. 7: SlCSN5-2 regulates accumulation of the SlBBX20 protein.

From: SlBBX20 interacts with the COP9 signalosome subunit SlCSN5-2 to regulate anthocyanin biosynthesis by activating SlDFR expression in tomato

Fig. 7

a The effect of SlCSN5-2 on ubiquitination of the SlBBX20 protein. The SlBBX20-HA and SlCSN5-2-FLAG plasmids were cotransformed into tobacco protoplasts. Anti-FLAG matrix beads were used for immunoprecipitation experiments. Finally, several antibodies (anti-HA, anti-FLAG, and anti-UBQ) were used to detect the accumulation of different proteins, and actin was used as a control. b Relative NbCSN5B expression level in NbCSN5B-VIGS plants. Tobacco leaves were infiltrated with pTRV2-NbCSN5B and pTRV2 as controls. c NbCSN5B downregulated the SlBBX20-HA protein. NbCSN5B was silenced in tobacco by virus-induced gene silencing. The protoplasts were extracted and assessed using anti-HA, TRV2 as a control

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