Fig. 3: Representative images of the phenotype, fold change in fresh biomass weight, and transcript abundance of the eight candidate reference genes measured by qPCR in the leaves of T. ravennae seedlings under salinity stress.
From: An optimized protocol for stepwise optimization of real-time RT-PCR analysis
Images were taken with a Canon EOS Rebel T6 digital camera and a 35 mm macro lens at 0, 10, 20, and 30 dpt with 0 (A), 150 (B), 300 (C) and 600 (D) mM NaCl. Fold change in fresh biomass weight (E) was calculated by dividing the fresh biomass weight at each time point by the fresh biomass weight at 0 dpt. RNA was extracted from the leaves at 0, 10, 20, and 30 days post treatment (dpt) with 0 (F), 150 (G), 300 (H), and 600 (I) mM NaCl. Ct values indicate the mean values of transcript abundance from three independent replicates ± standard errors (vertical bars)
