Fig. 5: The optimal number of reference genes for qPCR normalization in T. ravennae detected by geNorm.
From: An optimized protocol for stepwise optimization of real-time RT-PCR analysis
The pairwise variation (Vn/n+1) of the eight candidate reference genes under various conditions were calculated by the geNorm software, and the optimal number of reference genes was determined by the lowest number of genes with Vn/Vn+1 smaller than the threshold of 0.15
