Fig. 1: The phylogenetic tree of the RNase T2 gene family and the conserved gene sequence of candidate S-RNases in the genus Fragaria.

A The S-RNase genes with red, orange, and light blue backgrounds are from the tribe Amygdaleae, the family Solanaceae, and the tribe Maleae, respectively. The red circles represent Malus, and the yellow five-pointed stars represent Pyrus. The genes in red and green fonts are from F. viridis and F. vesca, respectively. Groups 1, 2, and 3 represent the three groups clustered together, which are marked with red, yellow, and green circular bands, respectively. Bootstrapping was performed with 1000 replicates. B Sa and Sb-RNase are closely related to the genus Prunus; therefore, 41 Prunus S-RNases (Supplementary Table S2) were used to compare and analyze the conserved S-RNase structure in F. viridis. Based on the S-RNase characteristics of Rosaceae35,37,38, the conserved S-RNase structure in F. viridis was divided further into C1–C3, RC4, and C5 as conserved regions and RHV as a hypervariable region. These regions are marked with thick lines