Fig. 3: Analysis of S-RNase expression by RT and qRT-PCR.

Lanes 1, 2, 3, 4, 5, 6, 7, 8, and 9 in A–D represent the flower balls, styles, ovaries, receptacles, pedicels, calyxes, petals, leaves, and anthers of F. viridis 42, respectively. Lanes 10, 11, 12, and 13 represent the flower balls of F. vesca 41, F. mandshurica 43, F. nilgerrensis 45, and F.× ananassa “Benihoppe”, respectively. A, B represent the expression of Sa and Sb-RNase based on specific primers. C Shows the expression results obtained using degenerate primers for Sa and Sb-RNase. D Represents the detection results of all cDNA samples with primers for a reference gene, EF-1α. E Illustrates the expression trends of S-RNase at different periods in styles after pollination. The abscissa represents the time after pollination, and the ordinate represents the level of expression