Fig. 5: Identification of the S-RNase genotype by polyacrylamide gel electrophoresis.

Each band indicated by a red arrow with the Sa tag is from Sa-RNase, and each band indicated by the a arrow with the Sb tag is from Sb-RNase. M represents the reference band for 300 bp and 400 bp markers. A1–101, B1–101, and D57–68 are the selfing progeny lines of “S1-02-S2-76-S3-09” (SaSb), and C1–45 and C71–94 are the selfing progeny lines of “S1-02-S2-30-S3-09” (SaSa). B46-70 are the selfing progeny lines of “S1-02-S2-76-S3-11” (SbSb). D1–56 are the hybrid progenies between “S1-02-S2-30-S3-09” (SaSa) and “S1-02-S2-76-S3-11” (SbSb). D69–97 are the selfing progeny lines of F. viridis 42 (0–3 generations). The serial number and order (from left to right and top to bottom) of the selfing lines are the same as those in Table 2