Table 1 Characteristics of the RNase T2 gene family in F. vesca and F. viridis

From: Molecular characteristics of S-RNase alleles as the determinant of self-incompatibility in the style of Fragaria viridis

Gene ID

Chromosome location

Intron number

pI

Molecular weight

Signal peptide

Domain size

Number of amino acids

Pattern 4

FvH4_4g31300.1

Chr4:30502482

7

5.38

102.0

N

>60%

916

CPSSNG

FvH4_4g31290.1

Chr4:30499449

3

5.23

25.7

Y23

>60%

229

CPSSNG

FvH4_2g17310.1

Chr2:14946893

7

8.17

27.6

Y28

>60%

245

—

FvH4_5g24800.1

Chr5:16134164

3

9.37

26.6

Y26

>60%

239

—

FvH4_1g10040.1

Chr1:5448902

1

8.08

26.0

Y19

>60%

230

—

FvH4_6g07740.1

Chr6:4649328

1

6.21

25.2

Y21

>60%

220

—

FvH4_6g07690.1

Chr6:4634036

1

6.28

26.2

Y15

>60%

226

—

FvH4_5g33850.1

Chr5:24565949

1

8.07

31.3

Y43

>60%

270

—

FvH4_1g19170.1

Chr1:11366424

1

8.95

22.7

Y23

>60%

198

—

FvH4_4g18130.1

Chr4:22047584

1

8.09

27.7

Y19

>60%

241

—

FvH4_2g25650.1

Chr2: 20739797

3

9.39

25.6

N

<60%

223

—

FvH4_6g22290.1

Chr6: 15948673

1

7.66

27.4

Y17

<60%

238

—

FvH4_2g25620.1

Chr2: 20733334

2

9.81

15.5

Y20

<60%

133

—

FvH4_5g24550.1

Chr5: 15908338

2

8.55

12.3

Y26

<60%

109

—

Unigene18150.1

Chr4:30504261

2

4.58

25.2

Y27

>60%

231

CPSSSG

Unigene13465.1

Chr4:30500729

3

5.35

25.8

Y23

>60%

229

CPSSNG

Unigene11523.1

Chr2:14950589

6

5.76

30.5

Y29

>60%

278

—

Unigene23139.1

Chr6:5067585

1

8.53

26.2

Y26

>60%

228

—

CL6424.Contig1.1

Chr1:5449834

1

8.57

25.8

Y19

>60%

229

—

Unigene10929.1

nr.

2

8.68

25.2

Y23

>60%

218

—

Unigene7320.1

nr.

2

8.55

25.6

Y24

>60%

221

—

Unigene9248.2

Chr5:24566583

0

5.47

12.4

N

<60%

104

—

Unigene638.1

Chr6:4649354

1

5.26

12.4

Y19

<60%

109

—

Unigene31475.1

Chr6:4634931

1

6.88

8.8

N

<60%

79

—

Unigene23536.2

Chr1:4771772

0

6.52

5.6

N

<60%

50

—

Unigene27236.1

Chr2:14949271

0

5.86

9.1

N

<60%

79

—

CL6424.Contig2.2

Chr1:5449478

1

8.94

8.5

N

<60%

75

—

  1. The genes with a “FvH4” prefix are from F. vesca, and those with “CL” or “Unigene” prefixes are from F. viridis. The “.1” suffix represents the first transcript of the gene in F. vesca, and the “.1” and “.2” suffixes represent the first and second open reading frames (ORFs) of the gene, respectively, in F. viridis. All identified genes of the RNase T2 family from F. vesca were consistent with the results of a previous screening43. The gene position and intron number for F. vesca were obtained according to the GFF file (F. vesca genome_v4.0.a1). The position information for F. viridis was determined mainly based on the F. vesca genome_v4.0.a1 reference and was located using BLASTn. Intron number and position analyses were based on the characteristic structure of the intron boundary (the intron analysis of only Unigene23139.1 refers to the F. nilgerrensis genome, v1.0). “nr.” means that the location information could not be obtained. In the signal peptide column, “N” indicates a lack of signal peptide information, “Y” indicates the presence of signal peptide information, and the number indicates the size of the signal peptide. The domain size is represented as a percentage, which is the percentage of the RNase T2 domain contained in the selected protein within the full-length domain. Pattern 4 (RNase T2 lineage amino acid pattern 4) is a type of amino acid sequence; if an RNase T2 family member contains the pattern 4, it is not an S-RNase or S-lineage gene18. The domain analysis of the RNase T2 family genes in F. vesca and F. viridis (see Supplementary Fig. S1) and chromosomal localization information are presented in Supplementary Figs. S2 and S3, respectively. The size of the gene and the gene structure are shown in Supplementary Fig. S4. The intron position and number for Unigene10929.1 and Unigene7320.1 were analyzed, as shown in Supplementary Figs. S5, S6, and Supplementary Dataset S1.
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