Fig. 5: Generation of T. antungense transgenic lines. | Horticulture Research

Fig. 5: Generation of T. antungense transgenic lines.

From: The basic helix-loop-helix transcription factor TabHLH1 increases chlorogenic acid and luteolin biosynthesis in Taraxacum antungense Kitag

Fig. 5

A Agrobacterium tumefaciens-mediated transformation generated transgenic T. antungense; (1) callus induction for treatment with recombinant Agrobacterium GV3101 carrying the TabHLH1-overexpressing construct, (2) transgenic callus, (3) shoot induction, (4) root induction, (5) well-rooted transgenic plant, (6) transplantation of transgenic plants. B Expression level of TabHLH1 in different transgenic lines. C Determination of polyphenol concentrations, including those of chlorogenic acid (CGA), caffeic acid (CA), rutin, and luteolin, by HPLC in T. antungense transgenic lines. D Expression levels of the key enzyme genes in the CGA and luteolin biosynthesis pathways in control and TabHLH1 overexpression transgenic lines

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