Table 1 Sequences of primers and probes used for the quantification of nptII copy number, and primer used for QPCR.
From: The Arabidopsis pattern recognition receptor EFR enhances fire blight resistance in apple
Primer and probe name | Sequence (5′–3′) |
|---|---|
MdTOPO6 | F: TGTGGAAGGAGATCAAAGCGCA R: CGCGTTGCTTCTTTGCTGCA |
NPTII | F: CTTGCCGAATATCATGGTGGAA R:GGTAGCCAACGCTATGTCCTGA |
MdTOPO6 probe | FAM-5′-ACATGCCAACAGGAACAATCACA-3′-TAMRA |
NPTII probe | FAM-5′-TTCTGGATTCATCGACTGTGGC-3′-TAMRA |
NPR1 | F-GATGAGAAAACCCCACTGGA R-TTGCTTTCGTCCATTTTTCC |
EFR | F-TTGTGGCTTCTCTGTGTTGG R-TTACCGAAATTGCCTGAACC |
MdUBI | F-CATCCCCCCAGACCAGCAGA R-CATCCCCCCAGACCAGCAGA |
MdACT | F-TGACCGAATGAGCAAGGAAATTACT R-TACTCAGCTTTGGCAATCCACATC |
MdWRKY25 | F-AATATACATTGGGAGCAAAAGAGTC R-AGAGTTCAGCATGGAAAGCG |
MdMPK3-2 | F-AGCAACCTCGTGTTGAATGC R-CCACAGACCACACGTCGATT |
