Fig. 2: Fine mapping of obv. | Horticulture Research

Fig. 2: Fine mapping of obv.

From: OBV (obscure vein), a C2H2 zinc finger transcription factor, positively regulates chloroplast development and bundle sheath extension formation in tomato (Solanum lycopersicum) leaf veins

Fig. 2: Fine mapping of obv.The alternative text for this image may have been generated using AI.

a Manhattan plot of GWAS for the obscure leaf vein. b–f Map-based cloning of the obv locus. Green arrow represents the most significant SNP association loci. b Green boxes represent the previous results of the fine positioning of obv. c Coarse linkage map of the obv locus on chr5. d The obv locus was narrowed down using 58 recombinants. e Three putative genes predicted in ITAG2.40. f OBV structure and the mutation site. Black boxes represent the coding sequences, and lines between boxes represent introns. g–h Relative expression levels of two genes in the leaf veins of IL-5-4-5-44 and M82 plants. A tomato ACTIN (Solyc03g078400) gene was used as the reference gene. Data are the mean ± SD of three biological replicates. Asterisks indicate statistically significant differences determined using a t-test (*P < 0.05; **P < 0.01)

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