Fig. 4: Phylogenetic relationships among RBOHs and relative PuRBOHF expression levels. | Horticulture Research

Fig. 4: Phylogenetic relationships among RBOHs and relative PuRBOHF expression levels.

From: Production of reactive oxygen species by PuRBOHF is critical for stone cell development in pear fruit

Fig. 4

a Phylogenetic tree of RBOH sequences of Arabidopsis thaliana (AtRBOH), Malus domestica (MdRBOH), and Pyrus ussuriensis (PuRBOH) based on a conservative approximate alignment. The scale indicates the genetic distance. b Heatmap of the qRT-PCR-derived cluster analysis of eight PuRBOH genes expressed in fruit flesh. c Relative transcript levels of PuRBOHD3 and PuRBOHF in stone cells and fruit flesh tissues at 40 days after full bloom (DAFB). d RNA in situ hybridization of PuRBOHF in fruit flesh tissues at 40 DAFB, cross-sectioned for hybridization with sense (left column) and antisense (right column) probes. Red triangles: in situ hybridization signals of PuRBOHF transcripts. e PuRBOHF expression in various tissues. f PuRBOHF expression under various abiotic stresses and hormone treatments. Error bars: standard deviations (three replications). *P < 0.05

Back to article page