Fig. 7: CmMYB012 binds to the promoters of CmCHS, CmDFR, CmANS, and CmUFGT to inhibit their transcription. | Horticulture Research

Fig. 7: CmMYB012 binds to the promoters of CmCHS, CmDFR, CmANS, and CmUFGT to inhibit their transcription.

From: A novel transcription factor CmMYB012 inhibits flavone and anthocyanin biosynthesis in response to high temperatures in chrysanthemum

Fig. 7: CmMYB012 binds to the promoters of CmCHS, CmDFR, CmANS, and CmUFGT to inhibit their transcription.

a Interactions of CmMYB012 proteins and the promoters of CmCHS, CmDFR, CmANS, and CmUFGT in yeast cells. pGADT7-GUS was used as a negative control. SD/-T/-H/-L indicates Trp, His, and Leu synthetic dropout media. 3-AT concentrations: 100 mM for CmCHSpro, 50 mM for CmDFRpro and CmANSpro, and 80 mM for CmUFGTpro. b Diagrams of effector and reporter vector constructs for the dual-luciferase assays. (c Graph showing the luminescence intensity. d–g Ratio of LUC to REN activity. The error bars indicate the SDs of six biological replicates. The samples denoted by different letters are significantly different (p < 0.01, ANOVA, Tukey test). Each point represents an independent calculation

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